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| 1. |  | GUERRA, M.P.; DAL VESCO, L.L.; PESCADOR, R.; SCHUELTER, A.R.; NODARI, R.O. Estabelecimento de um protocolo regenerativo para a micropropagacao do abacaxizeiro. Pesquisa Agropecuaria Brasileira, Brasilia, v.34, n.9, p.1557-63, set. 1999.| Biblioteca(s): Epagri-Sede. |
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| 2. | | GUERRA, M. P.; DAL VESCO, L. L.; DUCROQUET, J. P. H. J.; NODARI, R. O.; REIS, M. S. dos. Embriogenese somatica em goiabeira serrana: efeito do genotipo, choque auxinico e sementes sinteticas. Revista Brasileira de Fisiologia Vegetal, v.13, n.2, p.117-128, ago. 2001.| Biblioteca(s): Epagri-Sede. |
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| 3. | | SANDOVAL-YUGAR, E. W.; DAL VESCO, L. L.; STEINMACHER, D. A.; STOLF, E. C.; GUERRA, M. P. Microshoots encapsulation and plant conversion of Musa sp. cv. 'Grand Naine'. Ciência Rural, Santa Maria, v. 39, n. 4, p. 998-1004, jul. 2009.| Biblioteca(s): Epagri-Sede. |
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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
10/09/2020 |
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Data da última atualização: |
10/09/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Nacional - B |
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Autoria: |
SOUZA, J. A.; BOGO, A.; BETTONI, J. C.; COSTA, M. D.; SILVA, F. N.; CASA, R. T.; RUFATO, L. |
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Título: |
Droplet-vitrification cryotherapy for eradication of apple stem grooving virus and apple stem pitting virus from ?Marubakaido? apple rootstock. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, v. 45, n. , p. 148-152, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
The apple stem grooving virus (ASGV) and apple stem pitting virus (ASPV) are among the major viral species that infect apple trees and are responsible for substantial damage and loss. The aim of this study was to assess the effectiveness of droplet-vitrification cryotherapy technique in eradication of ASGV and ASPV in Malus prunifolia ?Marubakaido? apple rootstock plants. The axillary shoot tips were excised from ASGV- and ASPV-infected plants and incubated for one day on preculture medium. Subsequently, they were exposed to plant vitrification solution 2 (PVS2) for 0, 20, 40, 50, 60 or 80 min at 22 °C and immersed in liquid nitrogen for 1 h. Cryopreserved shoot tips had the highest survival (75 and 65%) and regrowth (53 and 58%) percentages with PVS2 exposure duration of 20 and 40 min, respectively. After six months of growth in a greenhouse, RT-PCR analyses revealed that all the tested plants were free of ASPV and 70% were free of ASGV. The high eradication percentage of ASGV and ASPV indicated that the droplet-vitrification cryotherapy technique is an effective tool to produce ASGV- and ASPV-free propagating material of ?Marubakaido? apple rootstock. |
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Palavras-Chave: |
Cryopreservation; Malus prunifolia; Propagation; Virus-free material. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01926naa a2200241 a 4500
001 1129905
005 2020-09-10
008 2020 bl uuuu u00u1 u #d
100 1 $aSOUZA, J. A.
245 $aDroplet-vitrification cryotherapy for eradication of apple stem grooving virus and apple stem pitting virus from ?Marubakaido? apple rootstock.$h[electronic resource]
260 $c2020
520 $aThe apple stem grooving virus (ASGV) and apple stem pitting virus (ASPV) are among the major viral species that infect apple trees and are responsible for substantial damage and loss. The aim of this study was to assess the effectiveness of droplet-vitrification cryotherapy technique in eradication of ASGV and ASPV in Malus prunifolia ?Marubakaido? apple rootstock plants. The axillary shoot tips were excised from ASGV- and ASPV-infected plants and incubated for one day on preculture medium. Subsequently, they were exposed to plant vitrification solution 2 (PVS2) for 0, 20, 40, 50, 60 or 80 min at 22 °C and immersed in liquid nitrogen for 1 h. Cryopreserved shoot tips had the highest survival (75 and 65%) and regrowth (53 and 58%) percentages with PVS2 exposure duration of 20 and 40 min, respectively. After six months of growth in a greenhouse, RT-PCR analyses revealed that all the tested plants were free of ASPV and 70% were free of ASGV. The high eradication percentage of ASGV and ASPV indicated that the droplet-vitrification cryotherapy technique is an effective tool to produce ASGV- and ASPV-free propagating material of ?Marubakaido? apple rootstock.
653 $aCryopreservation
653 $aMalus prunifolia
653 $aPropagation
653 $aVirus-free material
700 1 $aBOGO, A.
700 1 $aBETTONI, J. C.
700 1 $aCOSTA, M. D.
700 1 $aSILVA, F. N.
700 1 $aCASA, R. T.
700 1 $aRUFATO, L.
773 $tTropical Plant Pathology, Brasília$gv. 45, n. , p. 148-152, 2020.
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